Effect of the Combined Probiotics on Inhibiting Pathogenic Escherichia Coli Proiification

摘要

The study on the combined probiotics to replace antibiotics becomes more and more important because the by-effects and appearance of "superbug" from abuse of antibiotics in human life and animal production. In order to get the effective combined probiotics for replacing antibiotics and inhibiting pathogenic Escherichia coli (E. coli) proiification, 3 strains of Bacillus subtilis (B. subtilis, named as Bl, B2 and B3) were used to determine the pre-combination with experimental design (3 factorsX4 levels), and then the pre-combination of B. subtilis was combined with Lactobacillus casei (L. casei) and Hansenula anomala (H. anomala) to achieve the most effective combined probiotics with the same design. The results indicated that the pre-combination ratio of Bl, B2 and B3 was 0:1:1, and the most effective combination ratios of the pre-combination, L. casei and H. anomala were 0:1:1, 1:3:0, 2:2:3, 2:3:2, respectively (P<0.05). In addition, the incubating groups containing L. casei had better effect on inhibiting E. coli proiification than those without Lactobacillus, indicating that Lactobacillus had the best effect on inhibiting E. coli proiification, compared with other bacteria.