Extraction, purification, structural analysis and antioxidant activity of extracts from Alliums cepa L. var. agrogatum Don

摘要

The five extracts from Allium cepa L. var. agrogatum Don (ACAD) were determined for their total antioxidant activity by T-AOC kit. The highest antioxidant capacity of the extracts was ethanol fraction. Then the ethanol extracts were purified by AB-8 resin column and polyamide resin column successively. The refined samples were analyzed with HPLC by comparing with quercetin. Moreover, three different methods was selected for evaluating the antioxidant capacity of the reduction of scavenging activity of the stable DPPH radical, the hydrogen peroxide and superoxide anion 02-radical.Results shown that ACAD exhibited the highly efficient antioxidant activity in all the studied. Thus the results indicated that it could be used as functional food and antiatheroscloresis products.