Antiproliferative effects of licorice root flavonoids and dihydroquercetin in vitro

摘要

Flavonoids are polyphenolic compounds derived from plants which have large biological properties based on their ability to suppress proliferation. There is evidence that the basis of the mechanism of antiproliferative activity is the inhibition of phosphorylation of intracellular signaling molecules (kinases, transcription factors). The aim of this study was to compare antiproliferative effect of dihydroquercetin and licorice root flavonoids (LRF) against U-937 tumor cell line in vitro. Evaluation of proliferation of tumor cells was done using a radioisotope method. Proliferation was evaluated by ~3H-thymidine DNA incorporation assay. Radioactivity of ~3H-thymidine incorporated into DNA was measured after 24 h of cell culture incubation with flavonoids and the result was expressed as a percentage of control. These findings demonstrate that LRF and dihydroquercetin (0.1-20 mkg/ml) inhibit the proliferation of tumor cells in dose-dependent manner. However effect of dihydroquercetin was comparatively less pronounced than the effect of the LRF. Which is why, it can be concluded that LRF is more effective against growth of U-937 tumor cell line, than dihydroquercetin, and it might be perspective for the development of new anticancer agents.