Quantitative FRET measurement of the interaction of A_1 adenosine receptors and A_2a adenosine receptors in living cell

摘要

Adenosine plays important roles in the pain signal transduction by activating adenosine receptors of two subtypes of Ai and A_(2A). In this study, FRET system based independent emission -spectral spectral spectral unmixing method (Iem-spFRET) was set up and used to measure the energy transfer from A_1R to A_(2A)R. The energy transfer efficiency calculated by Iem-spFRET is about 17.44%. All the above date and results demonstrate that FRET with special designed fluorescence proteins could be used to investigate the interaction between adenosine receptors.