Optimization of crude extracts from Areca catechu L. flower using response surface methodology

摘要

Based on the single factor analysis, the effect of extraction time, extraction temperature, and solid to liquid ratio on enhancing crude extracts of essential oil yield from Areca catechu L. flowers was evaluated by response surface analysis (RSA). A three-variable, three-level Box-Behnken experimental design (BBD) was applied and the interactions of each factor on the essential oil crude extracts yield were also investigated. The test data were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed using the appropriate statistical methods. The optimal conditions were at 90 min extraction time, 60°C extraction temperature, and 15:1 solid to liquid ratio using petroleum ether with a boiling range of 60~90°C as the extraction agent. Under such conditions, the essential oil crude extracts yield is 2.52% after three runs.