Detection of 9α-OH-AD prepared by biotransformation

摘要

Detection of 9α-OH-AD prepared by biotransformation by RP-HPLC directly was studied. The detection is performed on a Kromasil 100-5C18 (4.6×250 mm) column, using methanol: water (7:3, v/v) as mobile phase, 0.8 mL·min~(-1) flow rate and external standard method, detected at 242 nm. There is a good line correlation between peak area and content in range of 0.01-0.20 g/L, the correlation coefficient is 0.9942, the average recovery is 99.09% with a relative stand deviation of 0.89% (n=5). The method is simple, stable, accurate and reliable for quality control of 9α-OH-AD.