The novel mTOR inhibitor RAD001 (Eeverolimus) induces antiproliferative effects in goat fetal fibroblasts

摘要

The mammalian target of rapamycin (mTOR) is an important controller of cell proliferation. RAD001 (everolimus) is a specific inhibitor of mTOR, can block mTOR signaling pathway. The purposes of this study are to explore the inhibitory effects of RAD001 on Cashmere goat fetal fibroblasts (GFb cells) proliferation. We examined both the effects of RAD001 on cell proliferation and cytostatic effect of GFb cells. The relative viability of the cells treated with RAD001 over 0.33 µM was significantly different compared with that of control (P < 0.01). As a result, RAD001 induced a dose-dependent decrease in cell proliferation. Taken together, these data showed that RAD001 can inhibit mTOR signaling and proliferation in GFb cells in vitro.