Effect of p-glucosidase and Gleditsia saponin on enzymatic hydrolysis process of furfural residues

摘要

Enzymatic hydrolysis constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition contributes to a high process cost. This study shows that appropriate concentration of Gleditsia saponin could reduce the enzyme loading and increase P-glucosidase active. The optimal conditions of enzymatic hydrolysis process of furfural residues was at enzyme loading of 6 FPU cellulose/g substrate and 9 IU ?-glucosidase/g substrate with 0.6 g/l of Gleditsia saponin. The enzyme loading could be reduced by 50% with addition of 0.64 g/1 Gleditsia saponin during furfural residues hydrolysis. The P-glucosidase activity from hydrolysate with 0.64 g/1 Gleditsia saponin was higher than that without any surfactants.