Study on the Isolation and Purification of Proanthocyanidins from Rhodiola rose by Macroporous Adsorbent Resin

摘要

Five different macroreticular resins (S-8, HPD750, AB-8, HPD100 and D101) were evaluated for the adsorption properties of the proanthocyanidins extracted from Rhodiola rose. The results showed that AB-8 displayed the optimal adsorption and desorption properties. The adsorption of AB-8 for proanthocyanidins followed Langmuir equation and belonged monolayer adsorption. The optimal purification conditions were as follows: the sample concentration of proanthocyanidins was 4mg/mL, sample flow rate was 1.5BV/h, proanthocyanidins could be entirely eluted with 3 bed volumes of 50% ethanol. Based on the experiment, the purity of proanthocyanidins product is 64%.