Determination of Fluoroquinolones Residual in Freshwater Fish by HPLC

摘要

HPLC (High Performance Liquid Chromatography) method for danofloxacin(DAN), enrofloxacin(ENR), sarafloxacin(SAR) and difloxacin(DIF) residues in freshwater fish was developed. The fish sample was extracted with methanol-water-acetic acid(2/8/0.01, V/V/V), performed on a C18 solid-phase extraction column. With methanol+malonic acid-magnesium chloride aqueous solution as mobile phase, gradient elution at 30°C, inject volume was 20uL. The wavelength of UV detector was 280 nm. The linear rang of four drugs was 0.1-10μg/mL. In the level of 0.5μg/kg and 4.0μg/kg, the average recovery of the four kinds of drugs was 79.81%~92.03% , the RSD was 1.03%~4.07%. The limit of DAN, ENR, SAR, and DIF was 0.1μg/kg, 0.1μg/kg, 0.05μg/kg, and 0.05μg/kg individual. This method was employed to analyze freshwater fishes, such as catfish, chub and carp, because of its simplicity and high efficiency.