Compared study on the microstructure and HPLC spectrum of radix isatis and rhizoma et radix baphicacanthis cusae

摘要

The microstructure and HPLC spectrum of Radix Isatis and Rhizoma et Radix Baphicacanthis cusae were studied. With the digital microscopy and HPLC, the microstructure and HPLC spectrum of commercially available Radix Isatis and Rhizoma et radix Baphicacanthis cusae from different sources were compared and analyzed. Compared Radix Isatis with Rhizoma et radix Baphicacanthis cusae from different sources, the results showed that the reticulated vessels, compound and single starch granules and stone cell were found in Radix Isatis, but there were the pitted vessels, parenchyma cells where there were small pits, single starch granules and cystolith in Rhizoma et radix Baphicacanthis cusae. Compared the similarity of HPLC spectrum, the results revealed that there were obvious differences between the microstructure and characteristic HPLC spectrum of Radix Isatis and Rhizome et radix Baphicacanthis cusae. The characteristic of the microstructure and HPLC spectrum of Radix Isatis and Rhizoma et radix Baphicacanthis cusae that was established in the study could be applied into the quality evaluation for these two medicines, at the same time provide a scientific basis and guarantee of methodology for quality specification.