Antioxidant Effect of Bighead Carp Protein Hydrofysates

摘要

The aim of this study was to observe the antioxidant effect of hydrolysates derived from bighead carp protein (HBCP). The effect was observed through the life span experiment of D. melanogasler and was evaluated by antioxidant indexes of D-galactose (D-gal) induced senile mice. The mean and maximum life span of D. meianagastcr with HBCP supplement in nutritional medium was compared to that of control group, which were fed without any addition. The senile mice were induced by ecliac injection of D-gal for six week. Experiment groups received HBCP at different dosages (3 g/kg, 1.5 g/kg and 0.5 g/kg) by intragastric administration, while the aged group received saline every day. Contents of maionadehide (MDA), activities of supcroxide dimutase (SOD) and glutathionc peroxidase (GSH-Px) were determined. The results showed that the addition of HBCP in feedstuff could increase the mean and maximum life span of D. melanogasler. Compared to the D-gal induced senile group, contents of liver and brain MDA in mice which received HBCP (3 g/kg and 1.5 g/kg) by intragastric administration were significantly decreased (p<0.01), while the activities of the antioxidative enzyme were increased. This result indicated that ] IBCP had an antioxidant effect for D-gal induced senile mice and can extend the life span of D. melanogasler.