Identification and primary application of two high-yield glucoamylase moulds from Luzhou-flavor Daqu

摘要

Six moulds were isolated from Luzhou-flavor Daqu through traditional microorganism isolation method. Among them, two strains njsys-3 and njsys-6 have shown higher glucoamylase activity, their enzyme activity were 1842 U/g and 2349U/g separately. Molecular biological identification results shown that strain njsys-3 was Aspergillus clavatus and strain njsys-6 was Aspergillus niger. Daqu-making test showed that once the inoculating amount of seeding broth of njsys-3 attached to 6 ‰, the glucoamylase activity of Daqu can increase 26.9%; when the inoculating amount of seeding broth of njsys-6 attached to 6 ‰, the activity of glucoamylase of Daqu can increase 34.1% and the fragrance of Daqu can increase.