Novel siRNA-loaded Bubble Liposomes with Ultrasound Exposure for RNA Interference

摘要

Recently, we have developed novel polyethyleneglycol (PEG) modified liposomes (Bubble liposomes; BLs) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure. We have shown that the combination of BLs and US was also useful for the delivery of siRNA. However, for use in intravenous administration, there is room for improvement in the colocalization of BLs and siRNA in blood vessels and the stability of siRNA. In this study, we have attempted to prepare novel siRNA-loaded BLs (si-BLs) using cationic lipid, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). As a result, siRNA loaded onto the surface of BLs could be observed. Furthermore, siRNA-loaded BLs were stable even in the presence of serum. The specific gene silencing effect caused by transfection with si-BLs and US could be also observed. Thus, si-BLs with US-exposure may be a useful novel transfection method for siRNA delivery to a target tissue or organ via systemic injection.