Immobilization of Porcine pancreatic Lipase on Vesicle-like Silica Material with Large Pore

摘要

Vesicle-like silica (VS) was prepared by utilizing cationic surfactant cetyltrimethylammonium bromide (CTAB) and anionic surfactant sodium dodecyl sulfate (SDS) as a dual-template. The mesostructures of VS were characterized by N_2 adsorption-desorption isotherms, field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). Results showed that mean mesopore size of unilayer and multilayer VS was about 14 nm and shell thickness was nearly 6 nm. The VS was chosen as support for immobilization of Porcine pancreatic lipase (PPL) by physical adsorption. The immobilized PPL possessed excellent thermal stability and reusability in the hydrolysis reaction of triacetin in phosphate buffer solution.