Toxicological Evaluation of Ionic Liquids Effect of Ionic Liquids on Human Colon Carcinoma HT-29 and CaCo-2 Cell Lines

摘要

Two colon carcinoma HT-29 and CaCo-2 cell lines were used as tools to investigate toxicity of several ILs on human intestinal epithelium. Negative results were obtained for some of the tested ILs which excludes their utilization when searching for human inoffensive ILs. If this is a priority, MIM cations with long alkyl chains will be putted aside, together with most of the Aliquat cations (Figures 1,2,3,4). Within the tested combinations, only [Aliquat] [NTf_2] is not toxic for CaCo-2 cells (Figure 4). Furthermore, DMG cation can also be harmful for human intestine when combined with some anions, such as PF_6 (Figure 6). Toxicity of these two last classes of cations were seen to depend greatly on the anion (Figure 4, 6). Amanzingly, NTf_2 anion changes completely cytotoxicity of Aliquat and DMG ILs: they are no longer cytotoxic to CaCo-2 cells dispite decreasing to a certain extent HT-29 cells viability (Figures 4,5,6,7). Similar result was also obtained for [P66614] [NTf2] which reduced the number of metabolic active HT-29 cells, without significant effect on CaCo-2 cells viability (Figure 6,7). Therefore, the presence of NTf2 reduces significantly CaCo-2 cytotoxicity and it seems to be acting as an anticarcinogenic agent in HT-29 cells.