Antimicrobial activity of extracts from different Origanum vulgare clones grown in Latvia

摘要

Origanum vulgare is widely used in pharmacies and folk medicine. Origanum vulgare clones grown in Latvia differ in their phytochemical content and morphological features. The aim of the study was to determine the antimicrobial activity of ten oregano clones grown at the Genetic resources collection of medical and herb plants at Latvia University of Agriculture. The antibacterial activity was assessed against bacteria (Esherichia coli, Pseudomonas aeruginosa, Staphylocoocus epidermidis, Bacillus cereus) and yeast (Candida albicans). A sensitivity tests were performed in the liquid nutrient media for bacteria and liquid malt media for Candida albicans. Plant ethanol extracts from leaves and flowers were added to the growth media. Extract and media proportion was 1:20. Growth of microorganisms was detected spectrophotometrically at wavelength 550 nm after 24 h of incubation at 28°C. Origanum vulgare leaves and flowers demonstrated different activity. The antimicrobial activity depends on O. vulgare clone. Various Origanum vulgare clones and plant parts showed different activity on used microorganisms.