Chemical Composition Antimicrobial activity In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

摘要

In this study, the essential oil of was evaluated for putative antibacterial activity against six clinical strains and five reference strains of , in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), -cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the strains. essential oil did not interfere with the neutralizing capacity of against the leukotoxin. In addition, it was shown that the EO had an antibacterial effect against on a similar level as some tested antimicrobials. In view of these findings, we suggest that EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to . In addition, it can be used together with the previously tested leukotoxin neutralizing .