Extended Gate Field Effect Transistor Based Measuring of the Vitamin C content of Orange Juice

摘要

An extended-gate field effect transistor (EGFET) of SnO{sub}2/ITO glass was applied to manufacture the vitamin C sensor. Therefore, we immobilized the ascorbate oxidase with 3-glycidoxypropyltrimethoxysilane (GPTS) method to measure the different concentrations of the vitamin C solution in an optimum measurement environment. In order to find the best measurement conditions of the biosensor, we studied the vitamin C sensor in different pH values of the phosphate buffer solution (PBS). Additionally, we used experimental results to discuss the response time and response voltage to compare vitamin C with orange juice for the vitamin C sensor.