Identification of Biologically Active Compounds from Nature Using Liquid Chromatography/Mass Spectrometry

摘要

Nature can serve as an excellent source of novel biologically active compounds. Unfortunately, a large number of known active compounds are often encountered during the search for novel chemistries. Therefore, early stage identification, and determination of novelty is of critical concern to natural product chemists. We have designed a screening process utilizing a single quadrupole LC/MS system to efficiently distinguish novel compounds from known compounds in crude biologically active extracts. This "dereplication" process is accomplished by chromatographing an extract on a LC column, diverting 95% of the eluent to a 96 well plate, and directing the remainder of the eluent to the MS for spectral analysis. Bioassay of the 96 well plate contents localizes the biologically active compounds, which are then structurally interrogated using MS, accurate MS, accurate MS/MS, and MS~n. These data are often sufficient to allow identification of the active compounds from the initial small scale extract, avoiding costly sample recollection or refermentation.