Gentle Microfluidic Cell Capture and Release: PLL Stabilization of the Secondary Anchor Targeted Cell Release System

摘要

Patients exhibit differential responses to drugs and therapeutics due to their unique genetic, proteomic, and cellular characteristics. Advancing personalized medicine thereby requires methods of isolating and profiling these differences to individualize therapeutic regimens. However, some current cell isolation techniques are slow, disrupting physiological receptor quantities while several of the more rapid 'lab-on-a-chip' methods apply force or chemical digestion to release selected cells, which causes irreversible changes to biomarkers. Thus, there is distinct lack of gentle and rapid cell isolation technologies for clinical translation. Towards these aims of translatable, fast and gentle cell isolation, we describe adaptation of our novel, Secondary Anchor Targeted Cell Release System (SATCR) to microfluidics and optimization of the microfluidic spiral design and buffer solutions to ensure gentle, but effective cell capture.