THE EFFECT OF CHITINASE AND 1,3-B-GLUCANASE GENESIN TRANSFORMED COTTON ON DISEASES

摘要

Two cotton lines were transformed with a bean chitinase (ch5b) and one of the lines with $-1,3 glucanase (bg). Individuals ofeach transformation were tested for presence of the gene, increased and tested in a greenhouse assay for activity againstRhizoctonia solani. The lines with the best apparent resistance were increased and tested in three replicated field trials, whichalso included the original parent and commercial variety Paymaster HS-26. At sites which was predominantly affected byThielaviopsis basicola, Meloidogyne incognita, and Rotylenchulus reniformis, there were no benefit or detriment of thetransformed genes on root necrosis or nematode population density. At a site dominated by Verticillium dahliae, several of thetransformed breeding lines to the more wilt susceptible parent became more resistant and one line became more susceptible ascompared to the nontransformed parent. For more wilt resistant parent,, transformed lines either had equal or higher susceptibilityto wilt than the nontransformed parent.