Spectral Karyotyping (SKY) in Hematologic Neoplasia

摘要

From November 1, 1997 till November 1, 2000 we have investigated 204 cases of acute myeloid leukemia (AML) (n = 95), acute lymphatic leukemia (ALL) (n = 40), myelodysplastic syndrome (MDS) (n = 11), chronic myeloid leukemia (CML) (n = 9), chronic lymphatic leukemia (CLL) (n = 4) and non-Hodgkin lymphoma (NHL) (n = 45) cytogenetically, using G-band analysis and spectral karyotyping (SKY). By SKY we were able to detect the abnormal clones in all cases but 9. In the G-band preparations these cases showed very few abnormal mitoses. The SKY either extended or confirmed the G-band findings in 94% of those with an abnormal karyotype. Cryptic translocations (translocations not suspected from the G-band karyotype) were found in 71 cases (26 AML, 9 ALL, 5 MDS, 2 CLL and 29 NHL). We find SKY a powerful adjuvant diagnostic tool that does not compromise one of the advantages of karyotyping techniques, the analysis of the entire genome which, in contrast to molecular biological techniques, still leaves the possibility to get more answers than questions posed.