On-line recovery of large molecules from mixture solution using semi -continuous size exclusion chromatography

摘要

The recovery of Blue Dextran from the mixture solution with vitamin B_(12) was carried out using the reciprocating size exclusion chromatography (RSEC). The separation performances in RSEC were compared with those in the conventional size exclusion chromatography (SEC) with recycle. The recovery yields of Blue Dextran in RSEC and SEC with recycle after five cycles were 64% and 51%, respectively.