We report several methods to monitor and improve reagent distribution and mixing on an acrylic card designed to demonstrate multiplexed distribution. Results show that the even distribution of a reagent from a common central well to multiple channels is strongly dependent on the chemical state of the microchannel surfaces. We have characterized the performance in acrylic microchannels treated by solution, polymer adsorption, and chemical vapor-phase processes. We also describe several pumping systems built on our multiplex distribution cards that utilize electroosmotic flow as the motivating mechanism. By coating the appropriate channels with charged species, these systems have been demonstrated as a method of reagent distribution.
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