Effect of rose bengal on immunoglobulin production by mouse B lymphoma, WEHI-279 cells

摘要

Effect of Rose Bengal (RB) on immunoglobulin (Ig) production by mouse B lymphoma WEHI-279 cells was examined. At 100 #mu#M, RB increased IgE concentration in the culture medium but decreased the levels of IgG and IgM. To examine whether RB affect to the secretion of IgE from WEHI-279 cells or its synthesis, intracellular and extracellular Ig levels were compared. Cytoplasmic IgE level in the cells treated with 100 #mu#M RB was higher than in the cells cultured without RB. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiment showed that the level of productive #epsilon# transcripts of RB-added group was higher than that of control but RB did not affect germ-line #epsilon# transcripts. These results suggested that RB enhancement of IgE was due to the increase of the Ig synthesis by WEHI-279 cells, neither to the increase of secretion activity of Ig from the cells nor to the enhancement of class-switching to IgE producing cell.