Bio-micromanipulation system for high throughput screening of microbes in microchannel

摘要

In this paper, we propose a novel methodology on high throughput screening of microbes. We have developed a prototype of Microchannel system for high throughput screening of Escherichia coli. Experimental demonstration of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers were carried out with Laser manipulator system. We discussed the basic strategies to improve the working efficiency and the operability of themicromanipulation and presented a new direction in this field. In experiments, we show that transportation and separation of Escherichia coli with electric field and optical radiation pressure is profitable in the future practical application for the high throughput screening of microbes.