Distribution of fluorescent probe molecules throughout the phospholipid membrane depth

摘要

A method to determine the distribution function shape of the luminescent probe molecules over biological membrane depth is suggested. It is based on: (1) the unhomogeneous model of the probe ensemble which permits its selection alteration selectively of the position and distribution of excited probe molecules by the wavelength of the excitation light; (2) the use of the nonradiative electronic energy transfer (NEET) mechanism from probe molecules in vesicules to the acceptor ones in a buffer solution. The distribution function of the donor-excited molecules for different wavelength of the excitation light was calculated. Using the spectra of probe molecules at different $lambda$-ex$/ and Lippert's equation the values of the dielectric constant of the membrane $epsilon were obtained for different regions.