Toxicity pharmacokinetics and photodynamic properties of chlorin e6

摘要

Toxicity, pharmacokinetics, and the tumor damage effect of chlorin e$-6$/ after light irradiation were studied. The results show that chlorin e$-6$/ LD50 value in C57Bl mice was 189 $POM 10 mg/kg, in non-inbred white rats it was 99 $POM 14 mg/kg 14 days after the agent iv injection. The concentration of chlorin e$-6$/ in blood, liver, kidney, spleen, and tumors (sarcoma M-1 and sarcoma 45) of the rats was determined by the fluorescence method 3, 6, 12, 18, 24, 48, and 72 hours after the agent iv injection at the dose of 10 mg/kg. For this purpose chlorin e$-6$/ was extracted from tissues by detergent triton X-100. The depth of necrosis spreading in tumor tissue was evaluated after chlorin e$-6$/ injection at the doses of 1 - 10 mg/kg and subsequent irradiation by a krypton laser with light energy density of 90 J/cm$+2$/, using the method of vital staining with Evans blue. It was found that depending on the agent dose and time interval between chlorin e$-6$/ injection and photoradiation, the depth of tumor necrosis varied from 4.0 to 16.6 mm in sarcoma M-1 and from 5.0 to 15.0 in sarcoma 45.