In-vivo acute toxicological studies of Vasconcellea pubescens A. DC. fruit extract against hepatic injury

摘要

Vasconcellea pubescens A. DC. or mountain papaya fruit was extracted by means of maceration. This research aims to evaluate acute toxicity test on white mice liver. The toxicity parameter will be determined on LD_(50), toxic effect symptoms, SGPT serum levels, and hepatic histology profile. The experiment used 25 male Swiss Webster albino mice. The mice were divided into five groups. It consists of negative control (CMC 0.25%), dose I (1.12 mg/20g BW), dose II (5.6 mg/20g BW), dose III (28 mg/20g BW), and dose IV (140 mg/20g BW). All groups were administered orally with Extract of mountain papaya (EMP). Observations on the number of deaths as well as symptoms of toxic effects arisen during the first 24 hours and continued for up to 14 days were done. The levels of SGPT, LD50, and hepatic histologic profiles of mice were measured on the 15th day. Analysis of SGPT levels was performed statistically using one way ANOVA. The results showed the LD50 value of hydro-alcoholic EMP was 1400 mg/kg, which is categorized as mild toxic. The biochemistry parameter of ALT analysis showed that there was a significant difference between the control group and all treatment groups (p ≤ 0.05). The ratio of liver weight to the bodyweight of mice showed that at doses of 1400 and 7000, there was a significant difference to the control group (p < 0.05). The histology observation of hepatic organs showed cell liver damage for all groups.