Measurement of oxygen consumption of Saccharomyces cerevisiae using Biochip-C under influenced of sodium chloride and glucose

摘要

This paper aims to investigate the effects of sodium chloride (NaCl) and glucose on a single strain of yeast Saccharomyces cerevisiae during the respiration period. A Biochip-C sensor was used to measure the oxygen consumption rate and calibrated with PbS solution of pH 7.3 and 2.3 M before the experiment, which has a reference voltage of 1840 mV. A 150 μL of S.cerevisiae solution with a density of 5·10~7 cells/ml was immobilized into the Biochip-C chamber and left for 600 s at the initial experiment. An amount of 100 μL sodium chloride and glucose with concentrations of 0.1 M, 0.2 M, and 0.3 M were added into the yeast solution and measured until the stationary phase. For every single oxygen consumption, the models were then simulated to predict their stationary phase. The results show the addition of glucose causes an increase in the sensor potential level by a maximum of 3.9 % from the reference represented a higher oxygen consumption, while salt decreases 2.39 % lower, which is shows an inhibit fermentation process and reaches the stationary phase at 1200 s. This work comprises a model study of respiration for the fermentation phase of yeast, especially food screening applications, and to detect the amount of sugar in food.