Photochemically-driven Methods for the in situ and Site-Specific Fabrication of Monolithic-based Electrochromatographic Microsystems

摘要

This paper reports on the in-situ synthesis of reactive polymeric monolith and its subsequent surface functionalization. Poly(N-acryloyloxysuccinimide-co-ethylene dimethacrylate) was prepared through free radical photopolymerization of the respective monomers. The grafting procedure is based on the preliminary grafting of alkyne units on the monolith surface and the further radical photoaddition of functional thiol molecules. Implementation of UV-driven polymerization and grafting methods enabled the locally controlled immobilization of generic monolith within the confines of the capillary channel, and the site-specific immobilization of ligands on the pore surface of the monolith. Our results suggest that such a synthesis methodology is promising for integrating multiple analytical modules within microstructures for on-line analysis.