Effect of Sposknikovan on Spleen lymphocytes in Traumatized Mice

摘要

AIM: To observe the effect of Sposknikovan on spleen in traumatized mice.METHODS: A total of 60 Kunming mice were randomly divided into three groups: normal group, model group and treatment group. Sposknikovan were administered once by intragastric administration (i.g.), lasting seven days. We stripped each group of mice, weighed spleen and calculated spleen index after an hour when the last administration, then stained by propidium iodide dye. We used FACSAria flow cytometry and ModFit software for cell cycle analysis of spleen cell proliferation index and the proportion of the spleen lymphocyte subsets. RESULTS: A total of 60 Kunming mice were analyzed. 1) compared with control group, spleen index of model group decreased significantly, while treatment groups were higher than control and model group. 2)Proliferation index of spleen cells in model group was lower than control group; treatment group could increase the proliferation index of mouse spleen cells. 3)Compared with control group, the ratio of CD3+ CD4+/CD3+CD8+ was decreased in model group. The ratio of CD3+CD4+/CD3+CD8+ in treatment group was increased compared with control and model group. CONCLUSION: Sposknikovan retrieved the ratio of CD3+CD4+/CD3+CD8+ and enhanced immune function of spleen in traumatized mice by promoting proliferation of lymphocytes in traumatized mice.