METHOD FOR MORPHOMETRIC EVALUATION OF IMMUNE THROMBOCYTOPENIA COURSE PREDICTION AFTER SPLENECTOMY BASED ON SPLEEN CD4+/CD8+ T-LYMPHOCYTES WEIGHTS RATIO

摘要

FIELD: medicine.;SUBSTANCE: to implement the invention, the weight of the removed spleen is determined, histologic diagnosis of the postoperative material samples is performed in a conventional manner with preparation of paraffin blocks. To identify and count CD4+ and CD8+ lymphocytes, immunohistochemical staining is performed in histological sections of the spleen. In the spleen histological sections, the number of CD4+ and CD8+ cells is counted, their weight is calculated and the mass index ratio (MIR) of CD4+/CD8+ T-lymphocytes is calculated. At CD4+/CD8+ T-lymphocyts MIR values 2.3, a refractory form of immune thrombocytopenia is predicted, and at ≥2.4, the favorable course of the disease is concluded.;EFFECT: use of the invention provides effective morphometric assessment of immune thrombocytopenia course after splenectomy.;2 ex