Chemical and Spectroscopic Characterization of Humic Substances Isolated from South-Bohemian Peat

摘要

The aim of this work was study chemical composition and structure of different HS. Object of our study were two samples HS which were isolated from South-Bohemian peat from the quarry Branná near T?eboň, Czech Republic. Isolation of HS was performed according to the procedure recommended by the IHSS. All samples of HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of HS were calculated from the absorbance values. Fluorescence coefficients (Milori index and HIX) of HS were calculated from the area of the emission spectra. Biological/autochthonous index (BIX) and fluorescence index (FI) of HS were calculated from the ratio of different emission intensities.