Minimising Experimental Time for Kinetic Parameter Identification using Surface Plasmon Resonance by Injecting multiple analytes

摘要

The emergence of surface plasmon resonance-based optical biosensors has facilitated the identification of kinetic parameters for various macromolecular interactions (interactions between an analyte and a ligand). To determine these parameters, normally, the ligand is immobilized on the biosensor surface and experiments with arbitrarily chosen periods of analyte and buffer injections, and varying analyte concentrations are performed. The periods of injection and the analyte concentrations could be optimized to reduce experimental time. On the other hand, there are some ligands that can interact with more than one analyte, and the current practice is to test their interactions individually. In this work, it is shown that by injecting multiple analytes at the same time, it is possible to reduce the experimental time needed for kinetic parameter identification.