Laser irradiation in the visible wavelength stimulates wound healing in vitro

摘要

Summary: Low intensity laser irradiation (LILI) has been applied to stimulate healing in a variety of conditions,such as diabetes. This study aimed to determine the biochemical and molecular responses to LILI in the visiblered spectrum. Various cell culture models, namely wounded, diabetic wounded and hypoxic/ischemic were used.Models were exposed to visible red laser light (632.8, 636 or 660 nm) at a fluence of 5 J/cm2. Post-irradiation,the effect on cells was studied. Laser irradiation was shown to have a positive effect on stressed cells in vitro.There was an increase in migration, cell survival and proliferation, mitochondrial activity, secondary messengersand collagen. A decrease in cytotoxicity, DNA damage and pro-inflammatory cytokines were also seen. LLLToffers an alternative wound healing therapy. At a biochemical level there was a positive effect on cells, withstressed cells being pushed into cell survival pathways.