A CTC-MICROSEPARATOR FOR ISOLATION OF CIRCULATING TUMOR CELLS USING LATERAL MAGNETOPHORESIS AND MAGNETIC NANOBEADS

摘要

This paper introduces a high-speed high-performance CTC-microseparator for isolation of circulating tumor cells (CTCs) from human peripheral blood. The isolation is achieved through lateral magnetophoresis [1], generated by a ferromagnetic wire array inlaid on a glass substrate, and magnetic nanobeads, specifically binding to epithelial-derived CTCs. From healthy peripheral blood spiked with CTCs, the CTC-microseparator isolated 78.7% of the spiked CTCs and its purity was >95%. The overall isolation procedure was completed within 10 min per 1 ml blood. In addition, RT-PCR amplifications using the isolated CTCs were successfully performed. From peripheral blood of patients in breast and lung cancers, CTCs were isolated and identified through an immunofluorescent image analysis. From the experiment, we observed that all the cells isolated by the CTC-microseparator are CTCs.