MEASURING RAPID BINDING KINETICS BY MICRO ION-SELECTIVE ELECTRODES IN DROPLET-BASED MICROFLUIDIC DEVICES

摘要

Rapid kinetics was measured by potentiometric method in the paper. Droplet-based microfluidic chips with ion-selective microelectrodes and pneumatic valves were used to measure the binding kinetics of RNA with Mg~(2+) and DNA with K~+. Phase separation channels were added to remove the oil before the aqueous droplets reached the electrodes. Phase separation channels were required to obtain stable signals by the electrodes. The total consumption of oligonucleotides was less than 20 μL for a single experiment. The reaction time measured was from 0.05 to 25 s. The microfluidic system developed here is applicable to the biochemical reactions involving electrochemically active reactants or products.