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>High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions
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High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions
Biodiesel is an eco-friendly renewable fuel that can be derived from microalgae. Maximization of biomass and lipid productivities are considered the main challenges for algal biodiesel production. Since conventional batch cultures are time-, space-, and reagent-consuming with many restrictions to apply many replicates, microfluidic technology has recently emerged as an alternative low-cost and efficient technology with high throughput repeatability and reproducibility. Different applications of microfluidic devices in algal biotechnology have been reported, including cell identification, sorting, trapping, and metabolic screening. In this work, Chlorella vulgaris was investigated by encapsulating in a simple droplet-based micro-array device at different light intensities of 20, 80, and 200 µmol/m2/s combined with different nitrate concentrations of 17.6, 8.8, and 4.4 mM. The growth results for C. vulgaris within microfluidic device were compared to the conventional batch culture method. In addition, the effect of combined stress of deficiencies in irradiance and nitrogen availability were studied to illustrate their impact on the metabolic profiling of microalgae. The results showed that the most optimum favorable culturing conditions for Chlorella vulgaris growth within the microfluidic channels were 17.6 mM and 80 µmol/m2/s.
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机译:生物柴油是一种环保的可再生燃料,可以从微藻类中提取。生物质和脂质生产率的最大化被认为是藻类生物柴油生产的主要挑战。由于常规的分批培养需要大量时间,空间和试剂,因此应用许多重复实验存在很多限制,因此微流体技术最近成为一种具有高通量可重复性和可重复性的低成本,高效技术。已经报道了微流体装置在藻类生物技术中的不同应用,包括细胞鉴定,分选,捕获和代谢筛选。在这项工作中,通过封装在简单的基于液滴的微阵列设备中,以不同的光强度20、80和200 µmol / m 2 sup / s结合不同的硝酸盐浓度对小球藻进行了研究。 17.6、8.8和4.4毫米。将微囊藻在微流控设备中的生长结果与常规分批培养方法进行了比较。此外,研究了辐照度不足和氮素利用率联合胁迫的影响,以说明它们对微藻代谢谱的影响。结果表明,在微流控通道内,小球藻生长的最佳最佳培养条件为17.6 mM和80 µmol / m 2 sup> / s。
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