a Preparation of monodispersed spore suspension. Sterile cotton and four, six, and eight layers of filter papers were tested for filtering the spore suspension. A monodispersed spore suspension was obtained after filtrated through eight layers of sterile filter paper. Scale bar: 5 μm. b Germination of monodispersed S. lividans 66 spores in R2YE liquid medium over 24 h. Scale bar: 10 μm. c Germination of S. lividans 66 spores in droplets over 24 h. Scale bar: 50 μm. d Optimization of droplet diameters at different flow rate ratios of oil-to-water phase. Scale bar: 100 μm. e Relationship between droplet diameters and the flow rate ratio of oil-to-water phase. The number in the circle indicates the droplet diameter, which was calculated from microscopy images using n = 100 droplets.
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机译：单分散孢子悬浮液的制备。测试无菌棉和四个，六个和八层过滤纸以过滤孢子悬浮液。在过滤通过八层无菌滤纸后获得单分散的孢子悬浮液。秤栏：5μm。 B在R2YE液体培养基中的Monodpersed S.的发芽单分散的S. 66孢子。秤栏：10μm。 C S. S.的萌发66个液滴在24小时超过24小时。秤栏：50μm。 D液滴直径的D液滴直径的优化在水 - 水相的不同流速比。秤栏：100μm。液滴直径与油水相流量比的e。圆圈中的数量表示液滴直径，该直径由使用n = 100滴的显微镜图像计算。
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