(00524)Fast and Comprehensive Analysis of Major use Antibiotics by UHPLC-High Resolution and High Mass Accuracy Hybrid Linear Ion-Trap-Orbitrap Mass Spectrometry

摘要

In this study, antibiotics of multiple classes (sulfonamides, quinolones, penicillins, macrolides, tetracyclines, pyrimidines)were selected to be accurately detected with modern chromatographic systems based on hybrid mass analyzers. For that purpose 13 antibiotics of major use were separated and detected with ultra-high performance liquid chromatography (UHPLC)high-resolution LTQ/Orbitrap mass spectrometry. The recent trend is focused toward the use of powerful high resolution MS detectors like Orbitrap which has become the technique of choice because of its high selectivity and sensitivity. Compounds were successfully identified in spiked samples from their accurate mass and LC retention times from the acquired full-scan chromatogram.