THE STUDY OF ANTIOXIDANT COMPONENTS IN GRAPEVINE SEEDS

摘要

The wine pomace is a biodegradable waste that cannot be disposed in landfills used for the municipal waste. The content of antioxidant components that occur in this waste material is rather interesting. This study deals with contents of individual antioxidant components, polyphenols compounds and with the antioxidant activity of wine pomace.The antioxidant activity was determined spectrometrically by using two principally different methods, viz. the DPPH antioxidant assay and the FRAP method. The content of total polyphenols compounds was determined by means of the Folin-Ciocalteu method. The content of resveratrol, rutin, quercetin, gallic acid, coumaric acid, catechin and epicatechin was determined by means of the RP HPLC-UV/VIS detection method.According to the results obtained it can be concluded that grapevine (Vitis vinifera) seeds contain a very high amounts of antioxidant components that show a beneficial effect on the human health. The content of polyphenols compounds was 6.529 mg.Lt~(-1). The antioxidant activities estimated by means of DPPH and FRAP methods were 5.849 mg.Lt~(-1) and 1.762 mg.Lt~(-1), respectively. Catechin was the most frequent antioxidant and its content was 478 mg.Lt~(-1).