Method for selecting a transgenic grapevine or component thereof having increased resistance to a grapevine fanleaf virus; a substantially pure protein, isolated nucleic acid molecule; a vector comprising an isolated nucleic acid molecule; a cell transformed with such vector and a transgenic plant or component thereof including such vector

摘要

The invention refers to the biotechnology, used in the agriculture and comprises a method for selecting a transgenic grapevine or component thereof resistant to the grapevine fanleaf virus, as well as a vector comprising an isolated nucleic acid molecule, a cell transformed with such vector and a transgenic plant including such vector.On the whole the invention provides a method for selecting a transgenic grapevine or component thereof resistant to the grapevine fanleaf virus, the method including the following steps: a) transforming a grapevine cell with a grapevine nepovirus coat protein nucleic acid molecule or fragment thereof capable of being expressed in the mentioned plant cell; b) regenerating a transgenic grapevine or component thereof from the plant cell; and c) selecting a transgenic grapevine or component thereof which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or component thereof to the fanleaf virus.The invention also refers to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a “Geneva” isolate of a grapevine nepovirus.