Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation

摘要

The potential of human mesenchymal stem cells (hMSC) to differentiate into various types of mesenchymal tissue such as chondrocytes makes them a potential cell source in cartilage tissue engineering. Because of the requirement of high cell amounts for the generation of cartilage implants or for the extensive experimental studies to investigate the culture parameters the initial cells have to be expanded which leads to high population doubling numbers. It is known that hMSC can differentiate into chondrocytes at least up to the 15th population doubling. To monitor the differentiation status the protein MIA (melanoma inhibitory activity) which is only synthesized by malignant melanomas and chondrocytes can be used. In this study the chondrogenic differentiation potential of hMSC beyond the 15th population doubling was investigated using MIA as chondrocyte marker. A chondrogenic potential of hMSC at higher population doubling numbers may be of interest due to the requirement of less frequently isolations of cells. Therefore hMSC were cultured in monolayer until the 37th population doubling. Cells of different passages were cultured as pellets for two weeks in transforming growth factor (TGF)-B3 containing differentiation medium. The MIA contents in medium of the last three cultivation days in each case were measured using a MIA-ELISA-kit. A significant difference between MIA content in medium of pellet and non stimulated monolayer reference cultures could be detected until the 32nd population doubling. In addition the hMSC were seeded at lower densities to investigate whether the cells may be expanded faster and with less amount of work due to higher population doubling numbers per passage. The reduced inoculation density led to an increased growth rate.