A New System for the Enrichment of Cell Subclones Secreting High Levels of IgG Using Magnetic Cell Sorting (MACS~R Technology)

摘要

In this study, we demonstrate the effect of MACS~R Separation on the diversification pattern of the specific production rate (SPR) of heterogeneous IgG-secredng cell populations. Magnetic separation prior to single-cell subcloning results in an effectively reduced frequency of low antibody-producing clones in comparison to populations without separation. Thereby, we were able to gain clones with higher SPRs (up to 85 pg/cell/day) which were not detectable in the non-separated population. Additionally it was possible to increase the SPR of two antibody-secreting cell populations by MACS~R Technology without additional sub-cloning by a factor of 4 and 37, respectively. A further magnetic enrichment step led to an up to 48-fold increase of the SPR compared to non-separated cells.