'Novel Scaffolds for Tissue Engineering -Nano-Structured Surfaces Promote Selective Cell Attachment and Cell Differentiation'

摘要

Nano-structured carbon and carbon-composite materials promote the selective attachment of different primary cells and additionally induce the differentiation of progenitor cells even in the absence of growth factors. NHOst (normal human osteoblasts) cultivated on material comprising carbon fibers in a carbon matrix (carbon-carbon-composite) exhibited a trabecular growth pattern after 7 days, whereas control cells cultivated on polymeric lab dish surfaces still remained in a fibroblast phenotype. Cells on nano-structured fibrous materials comprised typical marker profiles for osteogenic differentiation (alkaline phosphatase, osteocalcin, procollagen type I). When cultivated on standard lab dish surfaces NHOst showed no such typical expression of osteogenic marker molecules.In contrast, endothelial HUVEC (human umbilical vein endothelial cells) cultivated in the presence of fibrous textured material showed no cell attachment. Selective adherent HUVEC cultivation on a nano-structured carbon-composite material was enabled by providing a spherical nano-structure instead of the fibrous morphology of the material. This material showed significant selective attachment for HUVEC but not for osteoblasts.