Exploring the Sensitivity Differences for Targeted Peptide Quantification in the Low Flow Rate Regime

摘要

The flexibility and reproducibility of the ekspert nanoLC 400 system makes it an excellent LC system for labs performing proteomics workflows, from high sensitivity protein expression analysis to high throughput peptide quantitation. (1) User exchangeable flow modules enables both nanoflow experiments and microflow experiments to be used as the workflows demand; (2) MFCPlus Technology provides flow stability for highest retention time reproducibility, with retention time variability below 0.35% RSD at 500 nL/min; (3) The fast, high precision autosampler provided accurate, reproducible sample injections (<1% CV) even in μL-pickup mode with speeds compatible with high throughput microflow experiments. Microflow experiments can be performed with higher throughput and robustness with a small decrease (～3 - 4x) loss in sensitivity from the nanoflow experiments, making it a very attractive option for higher throughput, more robust quantitative experiments.