Top-Down Analysis of Intact Antibodies Using Orbitrap Mass Spectrometry

摘要

Orbitrap top-down analysis can be used as a powerful tool for the characterization of therapeutic mAbs in both native and denatured forms. Ultrahigh resolving power achieved for 3 - second detection time on a compact, high-field Orbitrap instrument, allowed to baseline isotopically resolve an 148 kDa intact antibody. Ultrahigh resolving power was used to resolve and unambiguously assign overlapping isotopic clusters from top-down analysis of an intact antibody.