Top-down protein analysis process for mass spectrometry involves fragmenting proton ions and part protein removal from ions to produce mixture for mass spectrum

摘要

The analysis process creates fragment ion spectra suitable for evaluation from highly-charged protein ions in mass spectrometers with ion traps. Fragmentation of the protein ions and also partial removal of the protein ion take place in the ion trap. This process is taken far enough to produce a mixture of simply-charged and multiple-charged fragment ions, which is taken as the basis for the mass spectrum.